Product Description

Magic SYBR Green qPCR mix uses SYBR Green I fluorescent dye to premix all reagents required for qPCR into 2 × Mix reagent can accurately and quantitatively detect the target gene only by adding templates and primers. It has the characteristics of high sensitivity, good specificity and good stability.

Product Features

High cost performance: save the experimental cost and ensure the experimental results

Easy to operate: 2 × Premix, easy to use.

Strong specificity: the new hot start Taq DNA polymerase can avoid the generation of non-specific products

Wide detection range: good standard curve can be obtained in a wide quantitative area.

Good repeatability: the cDNA sample was amplified and detected by multi hole qPCR, and the repeatability was good.

 

Figure 1: using mouse cDNA as template, 10 fold gradient dilution, amplification analysis with magic SYBR Green qPCR mix, 

The results show that magic SYBR Green qPCR mix has specific amplification and high amplification efficiency, and can obtain a good linear relationship in a wide linear range.

      

Fig. 2: target genes with different abundances were amplified with cabbage cDNA, human cDNA and mouse cDNA as templates respectively,

The results show that magic SYBR Green qPCR mix has specific amplification and high amplification efficiency. Reliable experimental data can be obtained in various types of template experiments.

Fig. 3: using mouse cDNA as template, 30 multiple wells were set. The results showed that magic SYBR Green qPCR mix amplified multiple wells with high repeatability.

Figure 4: freeze thaw magic SYBR Green qPCR mix for 5, 10 and 20 times, using mouse cDNA as template,

The results showed that after repeated freezing and thawing, the amplification performance of magic SYBR Green qPCR mix was not significantly different from that of the control (△ CT ≤ 0.2).

 Please click the link below to download the manual

M223 Magic SYBR Green qPCR Mix說明書V2.0.pdf